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US 8,012,693 B2Granted
Analysis of chemically crosslinked cellular samples
Assignee3M Innovative Properties Company
InventorsBathsheba E. Chong Conklin, Patrick J. Parks
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Abstract
A method of analyzing cellular samples that include a chemically crosslinked analyte is provided. The analysis typically involves the use of mass spectrometry.
Claims27
A method of analyzing analytes, the method comprising:
- providing a cellular sample comprising chemically crosslinked analytes, wherein the sample is one or more formalin-fixed, paraffin-embedded tissue sections, and wherein the analytes are proteins or peptides or combinations thereof;
- separating the chemically crosslinked analytes from the formalin-fixed, paraffin-embedded tissue sections by heat;
- reversing at least a portion of the chemical crosslinks in the crosslinked analytes and forming decrosslinked analytes, wherein said reversing at least a portion of the chemical crosslinks comprises cleaving at least a portion of the chemical crosslinks, with the proviso that substantially no naturally occurring bonds in the analytes
- are cleaved during said reversing at least a portion of the chemical crosslinks;
- and
- analyzing the analytes by analyzing the decrosslinked analytes using mass spectrometry.
3 more elements
A method of analyzing analytes, the method comprising:
- providing a formalin-fixed, paraffin embedded, tissue section comprising one or more analytes comprising chemically fixed bonds;
- removing the one or more analytes from the paraffin of the formalin-fixed, paraffin embedded, tissue section;
- cleaving at least a portion of the chemically fixed bonds in the one or more analytes and forming cleaved analytes, with the proviso that substantially no naturally occurring bonds in the analytes are cleaved;
- and
- analyzing the analytes by analyzing the cleaved analytes using mass spectrometry, wherein the one or more analytes are proteins or peptides or combinations thereof.
2 more elements
A method of analyzing an analyte, the method comprising:
- providing a cellular sample comprising a chemically crosslinked analyte, wherein the sample is a formalin-fixed, paraffin-embedded tissue section;
- removing the analyte from the paraffin of the formalin-fixed, paraffin embedded, tissue section;
- reversing at least a portion of the chemical crosslinks in the crosslinked analyte and forming a decrosslinked analyte, wherein substantially no naturally occurring bonds in the analyte are broken during the reversal;
- and
- analyzing the analyte by analyzing the decrosslinked analyte using mass spectrometry, wherein the analyte is a protein or a peptide.
2 more elements
Dependencies are inferred from the claim text.