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US 8,012,688 B2Granted
Gene expression profiling from FFPE samples
AssigneeLife Technologies Corporation
InventorsMark G. Erlander, Ranelle C. Salunga
Life of this patent
Estimating the term…
Abstract
Methods and compositions relating to the generation and use of gene expression data from tissue samples that have been fixed and embedded are provided. The data can be electronically stored and implemented as well as used to augment diagnosis and treatment of diseases.
Claims36
A method for global amplification and analysis of expressed RNA from cells of a deparaffinized FFPE (formalin fixed paraffin embedded) sample, said method comprising:
- synthesizing, with a primer comprising a sequence complementary to RNA extracted from said cells of said deparaffinized FFPE sample, to produce a plurality of first cDNA strands;
- amplifying said cDNA strands to produce amplified molecules;
- and
- hybridizing said amplified molecules to a microarray, wherein said hybridizing produces signals that reflect expressed RNA in said cells.
1 more element
A method for global amplification and analysis of expressed RNA from cells of a deparaffinized FFPE (formalin fixed paraffin embedded) sample, said method comprising:
- synthesizing, with a primer comprising a sequence complementary to RNA extracted from said cells of said deparaffinized FFPE sample, to produce a plurality of first cDNA strands;
- amplifying said cDNA strands to produce amplified molecules;
- and
- hybridizing said amplified molecules to a sortable array, wherein said hybridizing produces signals that reflect expressed RNA in said cells.
1 more element
Dependencies are inferred from the claim text.