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US 8,012,685 B2Granted
Detection of analytes and nucleic acids
AssigneeApplied Biosystems, LLC
InventorsMark E. Shannon, David W. Ruff
Life of this patent
Estimating the term…
Abstract
Methods of detecting at least one analyte and at least one nucleic acid in a sample are provided. Reagents for carrying out the methods are also provided.
Claims17
A method of detecting at least one non-nucleic acid target analyte and at least one target nucleic acid in a cell, comprising:
- a) lysing the cell in a multifunctional lysis buffer to produce a cell lysate;
- b) detecting at least one non-nucleic acid target analyte in the cell lysate using a proximity detection assay;
- and
- c) detecting at least one target nucleic acid in the cell lysate using a quantitative nucleic acid detection assay;
- wherein (b) and (c) occur in the same vessel.
2 more elements
A kit for detecting at least one target analyte and at least one target nucleic acid, comprising:
- at least one multifunctional lysis buffer comprising at least one chemical selected from NDSB-201, CHAPS, DEDTAB, Zwittergent 3-10, and CAPSO;
- and
- at least one ligase.
Dependencies are inferred from the claim text.