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AssigneeUniversity of Central Florida Research Foundation, Inc.
InventorsPappachan Kolattukudy, Tatiana Sirakova, Jaiyanth Daniel, Chirajyoti Deb
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A method of inducing latency in Mycobacterium permits preparation of an in vitro model system of latent mycobacterial infection. Latency is induced in a pure culture of Mycobacterium by exposing it to multiple stress conditions, including a low nutrient culture medium without glycerol, a low pH, a relatively high level of carbon dioxide and a relatively low gas phase oxygen level. An in vitro model of mycobacterial infection employs macrophages induced from THP1 cells which are then infected with Mycobacterium. The infected macrophages are grown under hypoxic conditions to induce latency in the mycobacteria. The in vitro model of infection is useful in evaluating compounds for activity against latent mycobacteria.
An in vitro model of latent tuberculosis, the model comprising an isolated culture of THP1 derived macrophages containing ingested Mycobacterium tuberculosis bacteria and incubated under hypoxic conditions of about 1% O2 and 5% CO2 for a time sufficient for the bacteria to accumulate increased lipids therein.
An in vitro model of latent mycobacterial infection, the model comprising an isolated culture of THP1 derived macrophages containing ingested Mycobacterium sp. cells and incubated under hypoxic conditions of about 1% O2 for a time sufficient for the bacteria to accumulate increased lipids therein.
An in vitro model of mycobacterial infection, the model comprising an isolated culture of THP1 derived macrophages infected with Mycobacterium sp. cells and incubated in a reduced oxygen atmosphere of about 1% so as to render the macrophages lipid-loaded.
A method of making a model of latent tuberculosis, the method comprising:
A method of making a model of mycobacterial infection, the method comprising:
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