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US 8,012,469 B2Granted
Universal GM-CSF expressing bystander human K562 cell line
AssigneeThe Johns Hopkins University School of Medicine
InventorsHyam I. Levitsky, Ivan Borrello
Life of this patent
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Abstract
The present invention provides a universal immunomodulatory cytokine-expressing bystander cell line, a composition comprising such a cell line and a cancer antigen, a method of making such a cell line, and a method of using such a composition.
Claims17
A universal bystander cell line, which:
- (i) is the human cell line K562 or a subclone thereof, which is modified to lack major histocompatibility class I (MHC-I) antigens and major histocompatibility class II (MHC-II) antigens, and (ii) is modified by introduction of a nucleic acid molecule comprising a nucleic acid sequence encoding an immunomodulatory cytokine operably linked to an inducible promoter, wherein the universal bystander cell line expresses about 500 ng or greater of the immunomodulatory cytokine/106 cells/24 hours upon being induced.
A composition comprising
- a universal bystander cell line, which:
- (i) is the human cell line K562 or a subclone thereof, which is modified to lack major histocompatibility class I (MHC-I) antigens and major histocompatibility class II (MHC-II) antigens, and (ii) is modified by introduction of a nucleic acid molecule comprising a nucleic acid sequence encoding granulocyte macrophage-colony stimulating factor (GM-CSF) operably linked to a promoter, wherein the universal bystander cell line expresses about 500 ng or greater GM-CSF/106 cells/24 hours;
- a cancer antigen;
- and
- an adjuvant.
2 more elements
Dependencies are inferred from the claim text.