Capillary electrophoresis apparatus and electrophoresis method

A capillary electrophoresis apparatus comprising:

• an exchangeable capillary;
• an optical irradiation system including a light source which irradiates the capillary with excitation light;
• an optical detection system which has a wavelength dispersion device and a two-dimensional detector, the wavelength dispersion device dispersing fluorescent light from the capillary, the two-dimensional detector detecting a fluorescence spectrum image acquired from the wavelength dispersion device;
• a capillary position measuring light source which irradiates the capillary with a capillary position measuring light when the capillary is exchanged;
• and
• a filter including a band pass filter or long-pass filter positioned on the output side of the capillary position measuring light source,
• wherein when the capillary is configured to be irradiated with the light from the capillary position measuring light source, the two-dimensional detector is configured to:
• detect a two-dimensional image of the capillary, obtain a plurality of fluorescence spectrum images corresponding to a plurality of positions on the capillary from a plurality of predetermined data acquisition areas on the two-dimensional detector, and correct a standard position of the fluorescence spectrum image which has been detected when the capillary was position at a standard position based on the position of the image of the capillary.

A capillary electrophoresis method in which a sample is electrophoresed in a capillary, the capillary is irradiated with excitation light emitted from a light source of an optical irradiation system, fluorescent light from the capillary is dispersed to generate a fluorescence spectrum, the fluorescence spectrum is detected by a two-dimensional detector, and the sample is analyzed based on the detection result,

• the capillary electrophoresis method including:
• irradiating the capillary with a light emitted from a capillary position measuring light source and passed through a filter including a band pass filter or long-pass filter when the capillary is exchanged;
• detecting an image of the capillary by the two-dimensional detector;
• setting a plurality of data acquisition areas on the two-dimensional detector based on the position of the image of the capillary;
• and
• detecting a plurality of fluorescence spectrum images corresponding to a plurality of positions on the capillary from the plurality of data acquisition areas on the two-dimensional detector.

A capillary electrophoresis method in which a sample is electrophoresed in a capillary, the capillary is irradiated with excitation light emitted from a light source of an optical irradiation system, fluorescent light from the capillary is dispersed to generate a fluorescence spectrum, the fluorescence spectrum is detected by a two-dimensional detector, and the sample is analyzed based on the detection result,

• the capillary electrophoresis method including:
• irradiating the capillary with a light emitted from a capillary position measuring light source and passed through a filter including a band pass filter or long-pass filter when the capillary is exchanged;
• detecting an image of the capillary by the two-dimensional detector;
• detecting a plurality of fluorescence spectrum images corresponding to a plurality of positions on the capillary from a plurality of predetermined data acquisition areas on the two-dimensional detector;
• and
• correcting a standard position of fluorescence spectrum image which has been detected when the capillary was positioned at the standard position based on the position of the image of the capillary.