Blood separating device
AssigneeBiomet Biologics, LLC
InventorJacy C. Hoeppner
Life of this patent
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Abstract
A device for separating components of a composition according to density. The device includes a rotatable chamber and a plurality of buoys. The rotatable chamber has an axis of rotation. The plurality of buoys are positioned within the rotatable chamber and about the axis of rotation. The buoys are radially movable between an expanded position in which the buoys are at a first location relative to the axis of rotation and a contracted position in which the buoys are at a second location relative to the axis of rotation. The first location is further from the axis of rotation than the second location is. The buoys are movable in response to force generated during rotation of the rotatable chamber.
Claims36
A method for separating whole blood into different components comprising:
- inserting whole blood into a rotatable chamber of a blood separation device having an axis of rotation, an inner wall surface, and a plurality of movable buoys between the axis of rotation and the inner wall surface;
- rotating the chamber for a sufficient period of time such that components of the whole blood separate according to density;
- and
- stopping rotation of the chamber such that the plurality of buoys move from an expanded position in which the buoys are distal to the axis of rotation to a contracted position in which the buoys are proximal to the axis of rotation to isolate red blood cells of the blood between the buoys and the inner wall surface, to isolate platelet poor plasma between the buoys and the axis of rotation, and to isolate platelet rich plasma at a common distance from the axis of rotation as the buoys.
A method for separating bone marrow aspirate into different components comprising:
- inserting bone marrow aspirate into a rotatable chamber of a bone marrow separation device having an axis of rotation, an inner wall surface, and a plurality of movable buoys between the axis of rotation and the inner wall surface;
- rotating the chamber for a sufficient period of time such that components of the bone marrow aspirate separate according to density;
- and
- stopping rotation of the chamber such that the plurality of buoys move from an expanded position in which the buoys are distal to the axis of rotation to a contracted position in which the buoys are proximal to the axis of rotation to isolate red blood cells of the bone marrow aspirate between the buoys and the inner wall surface, to isolate bone marrow plasma between the buoys and the axis of rotation, and to isolate multipotent cells at a common distance from the axis of rotation as the buoys.
A method for separating components of a multi-component biological mixture comprising:
- inserting the mixture into a rotatable chamber of a separation device including an axis of rotation, an inner wall surface, and a plurality of movable buoys between the axis of rotation and the inner wall surface;
- rotating the chamber for a sufficient period of time such that components of the mixture separate according to density;
- and
- stopping rotation of the chamber such that the plurality of buoys move from an expanded position in which the buoys are distal to the axis of rotation to a contracted position in which the buoys are proximal to the axis of rotation to isolate a first component of the mixture between the buoys and the inner wall surface, to isolate a second component of the mixture between the buoys and the axis of rotation, and to isolate a third component of the mixture at a common distance from the axis of rotation as the buoys.
A method for separating bone marrow aspirate into different components comprising:
- inserting the bone marrow aspirate mixture into a rotatable chamber of a separation device including an axis of rotation, an inner wall surface, and a plurality of buoys movable between the axis of rotation and the inner wall surface, the buoys are movable from an expanded position in which the buoys are distal to the axis of rotation to a contracted position in which the buoys are proximal to the axis of rotation, the bone marrow aspirate mixture is inserted with the buoys in the expanded position;
- rotating the chamber with a rotating base for a sufficient period of time such that components of the mixture separate according to density;
- and
- stopping rotation of the chamber such that the plurality of buoys move radially along an angled floor of the chamber that is between the axis of rotation and the inner wall surface, the floor is inclined from the axis of rotation to the inner wall surface, the buoys move radially from the expanded position to the contracted position in order to:
- isolate red blood cells of the mixture between the buoys and the inner wall surface;
- isolate bone marrow plasma between the buoys and the axis of rotation;
- and
- isolate multipotent cells at a common distance from the axis of rotation as the buoys.
Dependencies are inferred from the claim text.