Extended depth of field imaging for high speed object analysis
AssigneeAmnis Corporation
InventorsWilliam Ortyn, David Basiji, Keith Frost, Luchuan Liang, Richard Bauer, Brian Hall, David Perry
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AssigneeAmnis Corporation
InventorsWilliam Ortyn, David Basiji, Keith Frost, Luchuan Liang, Richard Bauer, Brian Hall, David Perry
Estimating the term…
A high speed, high-resolution flow imaging system is modified to achieve extended depth of field imaging. An optical distortion element is introduced into the flow imaging system. Light from an object, such as a cell, is distorted by the distortion element, such that a point spread function (PSF) of the imaging system is invariant across an extended depth of field. The distorted light is spectrally dispersed, and the dispersed light is used to simultaneously generate a plurality of images. The images are detected, and image processing is used to enhance the detected images by compensating for the distortion, to achieve extended depth of field images of the object. The post image processing preferably involves de-convolution, and requires knowledge of the PSF of the imaging system, as modified by the optical distortion element.
A method for generating a three-dimensional map of an object, wherein the three-dimensional map exhibits a first extended depth of field along a first axis and a second extended depth of field along a second axis, comprising the steps of:
A method for generating a stereoscopic view of an object, while there is relative motion between the object and an imaging system used to produce the stereoscopic view, wherein the stereoscopic view exhibits a first extended depth of field along a first axis and a second extended depth of field along a second axis, where the first axis and the second axis are not parallel, comprising the steps of:
An imaging system adapted to perform extended depth of field imaging of an object, to acquire a first extended depth of field image of the object using light moving away from the object in a first direction, and a second extended depth of field image of the object using light moving away from the object in a second direction, where the first and second directions are different, such that a three-dimensional model of the object can be generated using the first and second extended depth of field images, the system comprising:
An imaging system adapted to perform extended depth of field imaging of an object in order to produce a stereoscopic image of the object, while there is relative movement between the object and the imaging system, comprising:
Dependencies are inferred from the claim text.