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US 8,008,475 B1Granted
Method for isolating and purifying nucleic acids on surfaces
AssigneeQiagen GmbH
InventorsHelge Bastian, Simone Gauch, Uwe Oelmüller, Susanne Ullmann
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Abstract
The present invention involves a process for the isolation of nucleic acids on surfaces by means of at least the following steps: charging of a surface from a given direction with nucleic acids; immobilization of the nucleic acids on the surface; release of the immobilized nucleic acids from the surface; and removal of the released nucleic acids essentially in the direction of charging. Preferably the loading takes place from the top.
Claims70
A process for isolating nucleic acids comprising the following steps:
- charging a non-siliceous membrane from a given direction with nucleic acids, wherein said nonsiliceous membrane has two opposing sides;
- immobilizing the nucleic acids on one side of the non-siliceous membrane by binding the nucleic acids to said one side of the membrane in the presence of an immobilization buffer;
- releasing the immobilized nucleic acids from the non-siliceous membrane by applying an elution agent wherein the released nucleic acids do not pass through to the other side of the non-siliceous membrane;
- and
- removing the released nucleic acids from the same side of the non-siliceous membrane on which the nucleic acids were immobilized,
- wherein the released nucleic acids are removed without retrieving materials that have contacted the other side of said non-siliceous membrane, and wherein the membrane has pores that have a diameter of 1 μm to 50 μm.
3 more elements
A process for isolating nucleic acids comprising:
- (1) immobilizing nucleic acids on one side of a non-siliceous membrane by binding the nucleic acids to said one side of the membrane in the presence of an immobilization buffer, followed by
- (2) releasing the immobilized nucleic acids from the membrane by applying to the membrane an elution agent, wherein the eluted nucleic acids do not pass through to the other side of the non-siliceous membrane;
- and
- (3) collecting the released nucleic acids from the same side of the membrane on which the nucleic acids were immobilized;
- wherein the nucleic acids are collected without retrieving materials that have contacted said other side of said membrane;
- wherein the membrane comprises a material selected from the group consisting of nylon, polysulfone, polyether sulfone, polycarbonate, polyacrylate, acrylic acid copolymer, polyurethane, polyamide, polyvinyl chloride, polyfluorocarbonate, polytetrafluoroethylene, polyvinylidene fluoride, polyvinylidene difluoride, polyethylene tetrafluoroethylene copolymerisate, polyethylene chlorodifluoroethylene copolymerisate, and polyphenylene sulfide;
- wherein the membrane material is hydrophilic, hydrophobic, hydrophilisized, or hydrophobisized;
- and wherein the membrane has pores that have a diameter of 1 μm to 50 μm.
5 more elements
Dependencies are inferred from the claim text.