US 8,008,461 B2 · Carbonyl reductase, gene therefor and use thereof

Abstract

The present invention is to provide a process for efficiently producing an optically active alcohol including (R)-3-hydroxy-3-phenylpropanenitrile. One of the features of the present invention is a polypeptide having an activity of asymmetrically reducing 3-oxo-3-phenylpropanenitrile isolated from a microorganism belonging to the genus Candida to produce (R)-3-hydroxy-3-phenylpropanenitrile, DNA encoding the polypeptide and a transformant of producing the polypeptide. Another feature of the present invention is a process for producing an optically active alcohol such as (R)-3-hydroxy-3-phenylpropanenitrile by reducing a carbonyl compound such as 3-oxo-3-phenylpropanenitrile by use of the polypeptide or the transformant.

Claims11

Dependent claims

An isolated DNA selected from the group consisting of:

(a) an isolated DNA comprising the polynucleotide sequence of SEQ ID NO:
1;
and
(b) an isolated DNA that encodes a polypeptide with at least 90% amino acid sequence identity to SEQ ID NO:
2, and wherein said polypeptide has an activity of asymmetrically reducing 3-oxo-3-phenylpropanenitrile represented by the formula (1) below;
to produce (R)-3-hydroxy-3-hydroxy-3-phenylpropanenitrile represented by the formula (2) below;

3 more elements

Dependencies are inferred from the claim text.

Carbonyl reductase, gene therefor and use thereof

Life of this patent

Carbonyl reductase, gene therefor and use thereof

Life of this patent

Abstract

Claims11