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US 8,008,447 B2Granted
Crystallization of antibody or fragment thereof
AssigneeGenentech, Inc.
InventorsAnnette Marie Clasen Hagewiesche, Julie Fukami, Mary E. M. Cromwell, Rachel Bulotsky Dinges
Life of this patent
Estimating the term…
Abstract
The present disclosure concerns methods of crystallization and/or concentration of antibody or antibody fragments. The methods comprise contacting an antibody or antibody fragment with a solution comprising a salt of a divalent cation. Crystals and/or protein gels of antibody or antibody fragments are useful in compositions and formulations.
Claims13
A method for producing crystals of an anti-VEGF antibody comprising:
- a) contacting said anti-VEGF antibody with a solution that comprises about 5 to 120 mM ZnCl2 and about 1 to 100 mM sodium acetate buffer at a pH of about 4.7 to 5.7;
- and b) incubating the anti-VEGF antibody and the solution until crystals of the antibody are formed, wherein said anti-VEGF antibody is bevacizumab.
A method for producing crystals of an anti-VEGF antibody comprising:
- a) contacting said anti-VEGF antibody with a solution that comprises about 5 to 100 mM MgCl2 and about 1 to 100 mM Tris buffer at a pH of about 9.0;
- and b) incubating the anti-VEGF antibody-and the solution until crystals of the antibody are formed, wherein said anti-VEGF antibody is bevacizumab.
Dependencies are inferred from the claim text.