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AssigneeNational Institute of Advanced Industrial Science and Technology
InventorsJun Hirabayashi, Atsushi Kuno, Sachiko Nakamura, Noboru Uchiyama
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As a result of investigating the optimum conditions of methods for immobilizing proteins that interact with sugar chains onto a substrate, it was revealed that coating the surface of a slide glass with GTMS enables immobilization at a higher S/N ratio than conventionally possible. Moreover, by using a substrate to which a rubber with a number of holes was affixed to form a number of reaction vessels, and further by spotting lectins onto the substrate and washing with PBST, the weak interactions between sugar chains and lectins were successfully detected with improved sensitivity. In addition, by introducing an evanescent excitation-type scanner, it became possible to detect the interactions between lectins and sugar chains without washing away the probe solution.
A method for analyzing an interaction between a sugar chain and a protein that interacts with a sugar chain, wherein the method comprises the steps of:
A glass substrate coated with a compound comprising an epoxy group as an active group, onto which a protein that interacts with a sugar chain has been immobilized, and in which a number of reaction vessels have been formed by affixing a rubber having a number of holes onto the glass.
A method for analyzing an interaction between a sugar chain and a protein that interacts with a sugar chain, comprising:
Dependencies are inferred from the claim text.