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US 8,008,075 B2Granted
Stem cell aggregate suspension compositions and methods of differentiation thereof
AssigneeViaCyte, Inc.
InventorsChad Green, Xiaojie Yu, Anne Bang, Eugene Brandon, Olivia Kelly, Alan D. Agulnick, E. Edward Baetge, Kevin A. D'Amour, Thomas C. Schulz, Allan J. Robins
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Abstract
The present invention relates to methods for production of undifferentiated or differentiated embryonic stem cell aggregate suspension cultures from undifferentiated or differentiated embryonic stem cell single cell suspensions and methods of differentiation thereof.
Claims11
A method of generating hES-derived cell aggregates in suspension, comprising:
- (a) providing a culture of undifferentiated adherent hES cells;
- (b) disassociating the adherent hES cell culture of step (a) into a hES single cell suspension;
- (c) seeding the hES single cell suspension in a defined culture medium that supports undifferentiated growth of the hES cells, thereby forming a hES single cell suspension culture;
- (d) agitating the hES single cell suspension culture at low shear, wherein the hES single cells in the suspension form substantially uniform hES cell aggregates;
- and
- (e) contacting the hES cell aggregates in suspension with a differentiating culture condition wherein hES cell aggregates in suspension form hES-derived cell aggregates in suspension,
- thereby generating hES-derived cell aggregates in suspension.
4 more elements
Dependencies are inferred from the claim text.