Anti-Mpl antibodies
AssigneeChugai Seiyaku Kabushiki Kaisha
InventorsHiroyuki Tsunoda, Kiyotaka Nakano, Tetsuro Orita, Masayuki Tsuchiya, Yuichi Hirata
Life of this patent
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Abstract
Anti-human Mpl antibodies were isolated and purified. Anti-human Mpl diabodies and anti-human Mpl sc(Fv)2 were prepared using genetic engineering techniques and anti-human Mpl sc(Fv)2 was also humanized. The diabodies and sc(Fv)2 were assayed for TPO-like agonistic activity, and were found to have activities higher than those of anti-human Mpl antibodies, or activities equivalent to or higher than those of naturally-occurring human TPO ligand.
Claims33
A polynucleotide encoding a single-chain antibody that binds to the thrombopoietin (TPO) receptor (Mpl), wherein said antibody comprises two heavy chain variable regions and two light chain variable regions, and at least one of the heavy chain variable regions comprises a set of CDR1, CDR2 and CDR3 sequences selected from the group consisting of:
- (a) SEQ ID NOs:
- 27, 28, and 29;
- (b) SEQ ID NOs:
- 36, 37, and 38;
- and
- (c) SEQ ID NOs:
- 57, 58, and 59.
Dependencies are inferred from the claim text.