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US 8,008,057 B2Granted
Subtilases
AssigneeNovozymes A/S
InventorsAllan Svendsen, Stefan Minning
Life of this patent
Estimating the term…
Abstract
The present invention relates to methods for producing variants of a parent JP170 subtilase and of a parent BPN′ subtilase and to JP170 and BPN′ variants having altered properties as compared to the parent JP170/BPN′ subtilase.
Claims15
A variant of a JP170 type subtilase, said variant having at least 70% identity to SEQ ID NO:1 and comprising one or more disulfide bridges introduced by one or more of the following modifications:
- A4C+P222C, G21C+A86C, V26C+A265C, G57C+G105C, G74C+A229C, Q111C+N143C, G160C+S170C, A286C+V349C, A27C+A122C, A45C+G78C, V72C+P258C, G78C+A229C, D98C+G104C, Q111C+Y147C, G135C+G167C, R142C+P354C, V144C+A178C, G182C+P217C, A183C+G223C, A195C+Y225C, F271C+P279C, A287C+A430C, A293C+T310C, E322C+S428C, S324C+A332C, S327C+P424C, D352C+N397C, G355C+T362C, G291C+S314C, wherein the positions correspond to the positions of the amino acid sequence set forth in SEQ ID NO:1, and wherein the variant subtilase hydrolyzes Suc-Ala-Ala-Pro-Phe-paranitroanilide.
Dependencies are inferred from the claim text.