Variant Hypocrea jecorina CBH2 cellulases
AssigneeDanisco US Inc.
InventorsWolfgang Aehle, Frits Goedegebuur, Lydia Dankmeyer, Colin Mitchinson, Paulien Neefe, Brad Kelemen, Robert Caldwell, Pauline Teunissen
Life of this patent
Estimating the term…
Abstract
Described herein are variants of H. jecorina CBH2, a Cel6A enzyme. The present invention provides novel cellobiohydrolases that have altered thermostability.
Claims51
An isolated variant of a CBH2 cellulase comprising SEQ ID NO:2 or SEQ ID NO:95, wherein the variant consists one or more substitutions as compared to SEQ ID NO:2 or SEQ ID NO:95, respectively, wherein said one or more substitutions are selected, in the context of SEQ ID NO:95, from the group consisting of:
- V94E, P98L, G118P, M120L, M134(G/L/V), T142V, L144(G/R/S), M145L, T148Y, T154A, L179A, Q204E, V206L, S210(L/R), I212V, T214(M/Y), L215I, G231N, T232V, V250I, Q276L, N285Q, S291G, G308A, T312S, S316P, V323(L/N/Y), N325D, I333L, G334A, S343P, T349L, G360R, S380T, A381T, S386P, F411Y, S413Y, A416G, Q426E and A429T.
A fungal cellulase composition enriched in a variant of a CBH2 cellulase comprising SEQ ID NO:2 or SEQ ID NO:95, wherein the variant consists one or more substitutions as compared to SEQ ID NO:2 or SEQ ID NO:95, respectively, wherein said one or more substitutions are selected, in the context of SEQ ID NO:95, from the group consisting of:
- V94E, P98L, G118P, M120L, M134(G/L/V), T142V, L144(G/R/S), M145L, T148Y, T154A, L179A, Q204E, V206L, S210(L/R), I212V, T214(M/Y), L215I, G231N, T232V, V250I, Q276L, N285Q, S291G, G308A, T312S, S316P, V323(L/N/Y), N325D, I333L, G334A, S343P, T349L, G360R, S380T, A381T, S386P, F411Y, S413Y, A416G, Q426E and A429T.
A culture medium comprising a variant of a CBH2 cellulase comprising SEQ ID NO:2 or SEQ ID NO:95, wherein the variant consists one or more substitutions as compared to SEQ ID NO:2 or SEQ ID NO:95, respectively, wherein said one or more substitutions are selected, in the context of SEQ ID NO:95, from the group consisting of:
- V94E, P98L, G118P, M120L, M134(G/L/V), T142V, L144(G/R/S), M145L, T148Y, T154A, L179A, Q204E, V206L, S210(L/R), I212V, T214(M/Y), L215I, G231N, T232V, V250I, Q276L, N285Q, S291G, G308A, T312S, S316P, V323(L/N/Y), N325D, I333L, G334A, S343P, T349L, G360R, S380T, A381T, S386P, F411Y, S413Y, A416G, Q426E and A429T.
A method of treating wood pulp comprising contacting said wood pulp with a variant of a CBH2 cellulase comprising SEQ ID NO:2 or SEQ ID NO:95, wherein the variant consists one or more substitutions as compared to SEQ ID NO:2 or SEQ ID NO:95, respectively, wherein said one or more substitutions are selected, in the context of SEQ ID NO:95, from the group consisting of:
- V94E, P98L, G118P, M120L, M134(G/L/V), T142V, L144(G/R/S), M145L, T148Y, T154A, L179A, Q204E, V206L, S210(L/R), I212V, T214(M/Y), L215I, G231N, T232V, V250I, Q276L, N285Q, S291G, G308A, T312S, S316P, V323(L/N/Y), N325D, I333L, G334A, S343P, T349L, G360R, S380T, A381T, S386P, F411Y, S413Y, A416G, Q426E and A429T.
A method of converting biomass to sugars comprising contacting said biomass with a variant of a CBH2 cellulase comprising SEQ ID NO:2 or SEQ ID NO:95, wherein the variant consists one or more substitutions as compared to SEQ ID NO:2 or SEQ ID NO:95, respectively, wherein said one or more substitutions are selected, in the context of SEQ ID NO:95, from the group consisting of:
- V94E, P98L, G118P, M120L, M134(G/L/V), T142V, L144(G/R/S), M145L, T148Y, T154A, L179A, Q204E, V206L, S210(L/R), I212V, T214(M/Y), L215I, G231N, T232V, V250I, Q276L, N285Q, 291 G, G308A, T312S, S316P, V323(L/N/Y), N325D, I333L, G334A, S343P, T349L, G360R, S380T, A381T, S386P, F411Y, S413Y, A416G, Q426E and A429T.
Dependencies are inferred from the claim text.