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US 8,008,051 B2Granted
Butynol I esterase
AssigneeBASF SE
InventorsBernhard Hauer, Thomas Friedrich, Christoph Nübling, Rainer Stürmer, Wolfgang Ladner
Life of this patent
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Abstract
The invention relates to novel proteins from Pseudomonas glumae, having esterase activity, in particular butynol I esterase activity, to nucleic acid sequences coding therefore, to expression cassettes, vectors and recombinant microorganisms; to methods for preparing said proteins and to the use thereof for enzymic, in particular enantioselective enzymic, ester hydrolysis or transesterification of organic esters.
Claims14
A method for enantioselective ester hydrolysis using a protein, wherein
- a) the protein is contacted with a stereoisomer mixture of an optically active ester of the formula I
- R1—COO—R2 (I),
- in which
- R1 is a straight-chain or branched, unsubstituted or mono-substituted or polysubstituted C1-C10-alkyl, C2-C10-alkenyl, C2-C10-alkynyl,
- R2 is a straight-chain or branched, unsubstituted or mono-substituted or polysubstituted C1-C10-alkyl, C2-C10-alkenyl, C2-C10-alkynyl, C7-C15-aralkyl or a mononuclear or polynuclear, unsubstituted or monosubstituted or polysubstituted aromatic radical, and
- R1 and/or R2 include at least one asymmetric carbon;
- and
- b) the optically active compounds arising from the stereoselective hydrolysis of any of the stereoisomers and/or the non-hydrolyzed ester enantiomer are obtained from the reaction medium,
- and wherein the protein has esterase activity, and the protein comprises the amino acid sequence of SEQ ID NO.:
- 7, or comprises an amino acid sequence having at least 95% sequence identity with SEQ ID NO.:
- 7.
8 more elements
A method for enantioselective transesterification, wherein
- a) a stereoisomer mixture of an optically active alcohol of the formula II
- R2—OH (II),
- in which
- R2 is a straight-chain or branched, unsubstituted or mono-substituted or polysubstituted C1-C10-alkyl, C2-C10-alkenyl, C2-C10-alkynyl, C7-C15-aralkyl or a mononuclear or polynuclear, unsubstituted or monosubstituted or polysubstituted aromatic radical, and optionally has at least one asymmetric carbon;
- is contacted with an ester of the formula I
- R1—COO—R2 (I),
- in which
- R1 is a straight-chain or branched, unsubstituted or mono-substituted or polysubstituted C1-C10-alkyl, C2-C10-alkenyl, C2-C10-alkynyl,
- R2 is a straight-chain or branched, unsubstituted or mono-substituted or polysubstituted C1-C10-alkyl, C2-C10-alkenyl, C2-C10-alkynyl, C7-C15-aralkyl or a mononuclear or polynuclear, unsubstituted or monosubstituted or polysubstituted aromatic radical, and
- R1 and/or R2 include at least one asymmetric carbon;
- in the presence of a protein, and the unreacted alcohol stereoisomer is obtained from the reaction medium;
- or
- b) a stereoisomer mixture of an optically active ester of the formula I is contacted with an alcohol of the formula II in the presence of a protein, and a stereoisomer of the optically active alcohol contained in the ester is obtained from the reaction medium;
- wherein the protein employed in (a) and (b) has esterase activity, and the protein comprises the amino acid sequence of SEQ ID NO.:
- 7, or comprises an amino acid sequence having at least 95% sequence identity with SEQ ID NO.:
- 7.
13 more elements
Dependencies are inferred from the claim text.