US 8,008,049 B2Granted

DNA coding for polypeptide participating in biosynthesis of pladienolide

AssigneeEisai R&D Management Co., Ltd.

InventorsKazuhiro Machida, Akira Arisawa, Susumu Takeda, Masashi Yoshida, Toshio Tsuchida

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Abstract

The present invention provides polypeptides that participate in the biosynthesis of the pladienolide macrolide compounds, DNA that encodes these polypeptides and variants of this DNA, transformants that maintain all or a portion of this DNA or variant thereof, and a method of producing the pladienolide macrolide compounds using these transformants. More particularly, it provides an isolated pure DNA that contains at least one region encoding a polypeptide that participates in pladienolide biosynthesis; polypeptide encoded by this DNA; a self-replicating or integrated-replicating recombinant plasmid carrying this DNA; a transformant maintaining this DNA; and a method of producing a pladienolide, characterized by culturing this transformant on culture medium and collecting pladienolide from this culture medium.

Claims15

Dependent claims

A DNA that is isolated and pure, and that contains at least one region encoding a polypeptide that participates in pladienolide biosynthesis, said DNA comprising at least one nucleotide sequence selected from the nucleotide sequences defined in any of the following (1) to (3):

(1) nucleotide sequences defined in any of the following (a) to (g):
(a) the continuous nucleotide sequence from the base 8340 to base 27935 of SEQ ID NO:
1;
(b) the continuous nucleotide sequence from the base 28021 to base 49098 of SEQ ID NO:
1;
(c) the continuous nucleotide sequence from the base 49134 to base 60269 of SEQ ID NO:
1;
(d) the continuous nucleotide sequence from the base 60269 to base 65692 of SEQ ID NO:
1;
(e) the continuous nucleotide sequence from the base 65707 to base 66903 of SEQ ID NO:
1;
(f) the continuous nucleotide sequence from the base 68160 to base 66970 of SEQ ID NO:
1;
(g) the continuous nucleotide sequence from the base 69568 to base 68270 of SEQ ID NO:
1;
(2) a nucleotide sequence having at least 90% identity over the full length sequence with any of the nucleotide sequences defined in (1);
(3) a DNA encoding SEQ ID NO:2-8.

14 more elements

Dependencies are inferred from the claim text.

Facts

Application: 11/630,689Filed 19 Jul 2005
Priority: JP 2004-21127920 Jul 2004
Published: US 2009/0269820 A129 Oct 2009
Granted: 30 Aug 2011
Term ends: Not estimatedThe source records don’t support an estimate.

Classification9 codes

C12P 17/08
Oxygen as only ring hetero atoms: containing a hetero ring of at least seven ring members, e.g. zearalenone, macrolide aglycons
C07K 14/36
From bacteria: from Actinomyces; from Streptomyces
C12N 9/0071
Oxidoreductases: acting on paired donors with incorporation of molecular oxygen
C12N 9/0073
Acting on paired donors with incorporation of molecular oxygen: with NADH or NADPH as one donor, and incorporation of one atom of oxygen 1.14.13