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US 8,008,038 B2Granted
Methods for determining oxygen concentration with protoporphyrin IX
AssigneeAcademisch Medicsh Centrum bij de Universiteit van Amsterdam
InventorsEgbert G. Mik, Michiel Sinaasappel
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Abstract
Described are methods and devices for determining a concentration of oxygen in a compartment comprising exciting protoporphyrin IX.
Claims22
A method for determining a concentration of oxygen in a compartment, the method comprising:
- exciting protoporphyrin IX in the compartment, wherein protoporphyrin IX, when excited, exhibits a luminescence and/or transient absorption having a lifetime and wherein the lifetime of the luminescence and/or transient absorption is dependent on the oxygen concentration in the compartment,
- measuring the lifetime of the luminescence and/or transient absorption exhibited by protoporphyrin IX in the compartment, and
- correlating the lifetime of the luminescence and/or transient absorption with the concentration of oxygen in the compartment.
A method for determining the concentration of oxygen in a compartment, said compartment selected from the group consisting of a cell, an organelle, a mitochondrion, a tissue, an organ, a tumor, and microcirculation, the method comprising:
- exciting, in the compartment, protoporphyrin IX, wherein protoporphyrin IX, when excited, exhibits luminescence and/or transient absorption having a lifetime, wherein the lifetime of the luminescence and/or transient absorption is dependent on the oxygen concentration in the compartment,
- measuring the lifetime of the luminescence and/or transient absorption exhibited by protoporphyrin IX in the compartment,
- comparing the measured lifetime of the luminescence and/or transient absorption exhibited by protoporphyrin IX in the compartment with a reference, and
- correlating the measured lifetime of the luminescence and/or transient absorption exhibited by protoporphyrin IX compartment with the concentration of oxygen in the compartment.
1 more element
Dependencies are inferred from the claim text.