Tagged ligands for enrichment of rare analytes from a mixed sample
AssigneeCellective DX Corporation
InventorsAllyn Forsyth, Helen Barnes
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Abstract
Method of enriching specific cells from cellular samples are disclosed, comprising contacting in solution a cellular sample with affinity-tagged ligands (ATLs) each comprising a first ligand linked to an affinity tag, wherein the ligand selectively binds a cellular marker of the rare cells and the affinity tag can be selectively captured by a capture moiety, wherein the affinity tags do not comprise a magnetic particle; and flowing the sample through a microfluidic device comprising the capture moiety to selectively retain ATL-bound cells. Methods for enriching circulating tumor cells, and devices for enriching specific cells from cellular samples are also disclosed.
Claims9
A method for enriching rare cells from a cellular sample comprising:
- (a) contacting in solution said cellular sample with affinity-tagged ligands (ATLs) each ATL comprising:
- a first ligand linked to an affinity tag, wherein said ligand selectively binds a cellular marker of said rare cells, said affinity tag can be selectively captured by a capture moiety, and said affinity tag does not comprise a magnetic particle;
- and
- (b) flowing said cellular sample containing said ATLs through a microfluidic device comprising a substrate coated with said capture moiety to thereby selectively retain ATL-bound cells by binding said affinity tag to said capture moiety.
Dependencies are inferred from the claim text.