Method and device for characterizing cellular components of a biological fluid

A method of differentiating and counting cells present in a sample of a biological fluid, wherein the method comprises:

• (1) subjecting said sample to flow cytomeric analysis comprising measuring all of the cells present in the sample to be differentiated by electrical differentiation including impedance or an optical differentiation comprising diffraction, diffusion, transmission or fluorescence
• in a flow cytometer to obtain a set of primary results,
• (2) differentiating the cells of the sample into different populations and count the cells in each of the different populations, wherein the differentiating and counting are based on the primary results,
• (3) identifying the presence or absence of at least one anomaly in said set of primary results, wherein the anomaly is linked to an identified cellular peculiarity,
• (4) in response to identifying the presence of at least one anomaly identified in step (3), repeating the subjecting step in (1) and the differentiating step in (2) and further performing simultaneously steps (5) and (6) using the same aliquot of biological cell sample,
• (5) subjecting the sample to a further flow cytometric analysis in the flow cytometer, wherein the further flow cytometric analysis is based on the identified cellular peculiarity wherein the sample is tagged with a cell-specific dye or a cell-specific antibody conjugated to a dye or fluorochrome and the further flow cytometric analysis yields complementary results, and
• (6) differentiating the cells of the sample into different populations and/or subpopulations and count the cells in each of the different populations and/or subpopulations, wherein the differentiating and counting are based independently on the combination of the primary result, the repeated primary result, and the complementary result.