US 8,008,028 B2 · Panel cell used for granulocyte antibody detection

Abstract

A panel cell for detecting anti-HNA antibody is disclosed. The panel cell is obtained by introducing a DNA coding for an HNA antigen corresponding to the anti-HNA antibody into a cell so as to enable the expression of the DNA under the condition for use in the detection procedure, wherein the cell for DNA introduction exhibits no detectable reaction with anti-HLA-ABC antibody, anti-HLA-DR antibody, anti-HLA-DQ antibody, anti-HLA-DP antibody, anti-HNA-1 antibody, anti-HNA-2a antibody, anti-HNA-3a antibody, anti-HNA-4 antibody, anti-HNA-5 antibody, and serum from normal subject, in the detection procedure. The panel cell allows accurate and rapid detection of granulocyte antibody.

Claims11

Dependent claims

A method of detecting an anti-HNA antibody in a test sample, comprising the steps of:

(a) providing a test sample,
(b) providing a panel cell for detecting said anti-HNA antibody,
which is obtained by introducing a DNA coding for an HNA antigen corresponding to said anti-HNA antibody into a cell so as to enable the expression of said DNA under the condition for use in the detection procedure,
wherein the cell for DNA introduction is a K562 cell, and
(c) bringing said test sample into contact with said panel cell, and detecting the binding of said panel cell with said anti-HNA antibody.

2 more elements

Dependencies are inferred from the claim text.

Facts

Application

12/225,477Filed 13 Mar 2007

Priority

JP 2006-08123623 Mar 2006

Published

US 2009/0208978 A120 Aug 2009

Granted

30 Aug 2011

Term ends

Not estimatedThe source records don’t support an estimate.

Classification7 codes

G01N 33/6854
Involving proteins, peptides or amino acids: immunoglobulins
G01N 33/564
Immunoassay; Biospecific binding assay; Materials therefor: for pre-existing immune complex or autoimmune disease , i.e. systemic lupus…
C12N 2510/00
Genetically modified cells
G01N 2333/70539
Immunoglobulin superfamily, e.g. VCAMs, PECAM, LFA-3: mHC-molecules, e.g. HLA-molecules

Panel cell used for granulocyte antibody detection

Life of this patent

Panel cell used for granulocyte antibody detection

Life of this patent

Abstract

Claims11