Natural ligand of G protein coupled receptor RCC356 and uses thereof
AssigneeChemcom S.A.
InventorsFrédéric Sallman, Alex Veithen, Magali Philippeau
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Abstract
The invention relates to the identification of isovaleric acid as a natural ligand of the RCC356 G-protein coupled receptor (GPCR). The invention encompasses the use of the interaction of RCC356 polypeptides and isovaleric acid as the basis of screening assays for agents that modulate the activity of the RCC356 receptor. The invention also encompasses diagnostic and other assays performed based upon the RCC356/isovaleric acid interaction, as well as kits for performing diagnostic and screening assays.
Claims11
A method of identifying an agent that binds to a RCC356 polypeptide, wherein said RCC356 polypeptide comprises SEQ ID NO:
- 5, said method comprising:
- a) contacting said RCC356 polypeptide with isovaleric acid in the presence or in the absence of a candidate modulator under conditions permitting the binding of said isovaleric acid to said RCC356 polypeptide;
- wherein said contacting is performed in or on a cell expressing said RCC356 polypeptide, performed in or on synthetic liposomes, or performed in or on virus-induced budding membranes containing said RCC356 polypeptide polypeptide, and
- b) measuring the binding of said RCC356 polypeptide to said isovaleric acid, wherein a decrease in binding in the presence of said candidate modulator, relative to the binding in the absence of said candidate modulator, identifies said candidate modulator as an agent that modulates the function of said RCC356 polypeptide.
Dependencies are inferred from the claim text.