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US 8,007,998 B2Granted
Polymerized conjugates for biological applications
AssigneePierce Biotechnology, Inc.
InventorsSurbhi Desai, Michael Stanaitis, Ramesh Ganapathy
Life of this patent
Estimating the term…
Abstract
Polymerized conjugates of at least two proteins that have increased sensitivity for detecting proteins in biological assays such as gels and blots, and methods of preparing the conjugates, and a protein assay detection kit using the conjugates. In one embodiment, proteins are polymerized by reacting a carbohydrate group on the protein that has been oxidized with an amine group.
Claims15
A polymerized indicator-binding compound complex composition comprising
- a first polymer indirectly linked to a second polymer, where the first polymer comprises at least a first indicator molecule directly linked to a second indicator molecule and at least one binding compound directly linked to at least one indicator molecule, and where the second polymer comprises at least a third indicator molecule directly linked to a fourth indicator molecule, and where at least one indicator molecule of the first polymer is indirectly linked to an indicator molecule of the second polymer, wherein directly linked molecules comprise covalent bonds between the molecules, and indirectly linked molecules comprise covalent bonds between the molecules and a linker moiety, and
- at least one biocompatible excipient,
- wherein the composition is a polymerized indicator-binding compound complex.
A method of forming a polymerized indicator-binding compound complex, the method comprising
- (a) forming a polymerized, activated indicator-binding compound composition by reacting an indicator with at least a first activation agent under conditions to result in an activated indicator, and thereafter adding a binding compound to the activated indicator, under conditions to result in a polymerized, activated indicator-binding compound composition,
- (b) separately reacting an indicator with at least a second activation agent under conditions to result in a polymerized, activated indicator compound,
- (c) thereafter reacting the polymerized, activated indicator-binding compound composition from step (a) with the polymerized, activated indicator compound from step (b) under conditions to form a polymerized indicator-binding compound complex, wherein the polymerized indicator-binding compound complex comprises at least one indicator directly linked with another indicator, and at least one indicator directly linked with a binding compound.
A kit comprising
- a polymerized indicator-binding compound complex composition comprising a first polymer indirectly linked to a second polymer, where the first polymer comprises at least a first indicator molecule directly linked to a second indicator molecule and at least one binding compound directly linked to at least one indicator molecule, and where the second polymer comprises at least a third indicator molecule directly linked to a fourth indicator molecule, and where at least one indicator molecule of the first polymer is indirectly linked to an indicator molecule of the second polymer, wherein directly linked molecules comprise covalent bonds between the molecules, and indirectly linked molecules comprise covalent bonds between the molecules and a linker moiety, and
- instructions for using the polymerized indicator conjugate composition.
Dependencies are inferred from the claim text.