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US 8,007,996 B2Granted
Methods to identify protein arginine deiminase 4 inhibitors
AssigneeUniversity of South Carolina
InventorsPaul R. Thompson, Bryan Knuckley
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Abstract
In accordance with one embodiment of the present disclosure, a method to identify a protein arginine deiminase 4 inhibitor is disclosed. The method includes performing a competitive assay in which a potential inhibitor compound competes with rhodamine-conjugated fluoroamidine to bind to protein arginine deiminase 4. Fluorescence is measured to determine an estimate of the amount of fluorescent protein arginine deiminase 4 that is present in the assay.
Claims4
A method to identify a protein arginine deiminase 4 inhibitor comprising:
- performing a control assay in which rhodamine-conjugated fluoroamidine binds to protein arginine deiminase 4;
- measuring fluorescence to determine an estimate of the amount of fluorescent protein arginine deiminase 4 that is present in the control assay;
- performing a competitive assay in which a potential inhibitor compound competes with rhodamine-conjugated fluoroamidine to bind to protein arginine deiminase 4;
- and
- measuring fluorescence to determine an estimate of the amount of fluorescent protein arginine deiminase 4 that is present in the competitive assay;
- determining if the potential inhibitor compound is likely to act as an inhibitor of protein arginine deiminase 4 based on the estimate of the amount of fluorescent protein arginine deiminase 4 that is present in the competitive assay, wherein a decrease in fluorescence in the competitive assay when compared to the control assay is indicative that the potential inhibitor compound is a protein arginine deiminase 4 inhibitor.
3 more elements
Dependencies are inferred from the claim text.