Antibiotics GE 81112 factors A, B, B1, pharmaceutically acceptable salts and compositions, and use thereof

#The present invention concerns an antibiotic substance of microbial origin, arbitrarily denominated GE 81112 factor A, GE 81112 factor B and GE 81112 factor B1, a mixture of said factors in any proportion, the pharmaceutically acceptable salts and compositions thereof, and their use as an antibacterial agent.

#Another object of the present invention is a process for preparing GE 81112 factor A, GE 81112 factor B and GE 81112 factor B1, a mixture of said factors in any proportion, hereinafter reported as GE 81112 compounds or GE 81112 antibiotics.

#FIG. 1 provides the MsMs spectrum of GE 81112 factor A after fragmentation of the monoprotonated ion at m/z=644 with normalized collisions energy of 25%

#FIG. 2 provides the infrared spectrum of GE 81112 factor A in nujol.

#FIG. 3 provides 1H spectrum of GE 81112 factor A in DMSO-d6 and TFA (drops).

#Streptomyces sp. DSMZ 14386 originally designed with the internal code GE 81112 was isolated from a soil sample and deposited on Jul. 16, 2001, with the DSMZ, (Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Mascheroder Weg 1b, D-38124 Braunschweig, Germany), under the provision of the Budapest Treaty. The strain was accorded accession number DSMZ 14386.

#The production of GE 81112 compounds is achieved by cultivating a Streptomyces strain capable of producing them, i.e. Streptomyces sp. DSMZ 14386 or a variant or mutant thereof maintaining the genetic ability to produce said GE 81112 compounds; isolating the resulting antibiotic from the culture broth; purifying the isolated antibiotic; and separating the antibiotic three factors A, B and B1 or their mixture by chromatographic means. In any case, it is preferred to produce compounds GE 81112 under aerobic conditions in an aqueous nutrient medium containing assimilable sources of carbon, nitrogen, and inorganic salts, purifying the resulting compounds by means of chromatographic techniques. Many of the nutrient media usually employed in the fermentation field can be used, however certain media are preferred.

#Preferred carbon sources are glucose, xilose, starch, fructose, glycerol, and the like. Preferred nitrogen sources are soybean meal, peptone, meat extract, yeast extract, tryptone, aminoacids, hydrolized casein and the like. Among the inorganic salts which can be incorporated in the culture media, there are the customary soluble salts capable of yielding sodium, potassium, iron, zinc, cobalt, magnesium, calcium, ammonium, chloride, carbonate, sulphate, phosphate, nitrate, and the like ions.