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US 8,003,359 B2Granted
Eukaryotic amadoriase having excellent thermal stability, gene and recombinant DNA for the eukaryotic amadoriase, and process for production of eukaryotic amadoriase having excellent thermal stability
AssigneeKikkoman Corporation
InventorsKozo Hirokawa, Atsushi Ichiyanagi
Life of this patent
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Abstract
Disclosed are: a eukaryotic amadoriase which is prepared by introducing a mutation into DNA encoding a eukaryotic amadoriase derived from a microorganism belonging to the genus Coniochaeta or Eupenicillium so as to introduce a substitution into a specific amino acid residue in the eukaryotic amadoriase, thereby overcoming the defect associated with thermal stability; a gene or recombinant DNA for the eukaryotic amadoriase; and a process for production of a eukaryotic amadoriase having excellent thermal stability.
Claims4
An eukaryotic amadoriase, consisting of SEQ ID NO:1 with the exception of:
- (a) replacement of asparagine at position 272 of SEQ ID NO:1 by aspartic acid;
- (b) replacement of histidine a position 302 of SEQ ID NO:1 by arginine;
- and
- (c) replacement of histidine at position 388 of SEQ ID NO:1 by tyrosine.
1 more element
Dependencies are inferred from the claim text.