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US 8,003,340 B2Granted
Method and kit for isolating and fractioning phosphoproteins
AssigneesDCD, Ltd., Genomine, Inc.
InventorsDong Su Kim, Hyung Jin Na, Jae Ho Jang, Hye-Kyung Kim, Mo Yoel Park
Life of this patent
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Abstract
Disclosed herein a method and a kit for fractioning/isolating phosphoproteins. Calcium ions, barium ions, cobalt ions and molybdenum ions are bound specifically to phosphoproteins to form metal-phosphoprotein complexes. They can be easily precipitated with precipitants including sulfates, citrates, bicarbonates and carbonates.
Claims18
A method for fractioning phosphoproteins, comprising:
- (a) lysing cells or tissues in a lysis solution to afford a cell or tissue lysate;
- (b) adding a metal ion to the cell or tissue lysate to bind the metal ion with phosphoproteins of the cell or tissue lysate to form a metal-phosphoprotein complex, the metal ion being selected from a group consisting of calcium ions, barium ions, cobalt ions, molybdenum ions, and combinations thereof;
- (c) precipitating the metal-phosphoprotein complex with a precipitant selected from a group consisting of sulfate, citrate, bicarbonate and carbonate, to form a precipitate;
- and
- (d) separating the precipitate.
2 more elements
A kit for fractioning phosphoproteins, comprising:
- (a) lysing cells or tissues in a lysis solution to afford a cell or tissue lysate;
- (b) adding a metal ion to the cell or tissue lysate to bind the metal ion with phosphoproteins of the cell or tissue lysate to form a metal-phosphoprotein complex, the metal ion being selected from a group consisting of calcium ions, barium ions, cobalt ions, molybdenum ions, and combinations thereof;
- (c) precipitating the metal-phosphoprotein complex with a precipitant selected from a group consisting of sulfate, citrate, bicarbonate and carbonate, to form a precipitate;
- and
- (d) a means for separating the precipitate.
2 more elements
Dependencies are inferred from the claim text.