Multiplex cellular assays using detectable cell barcodes
AssigneeThe Board of Trustees of the Leland Stanford Junior University
InventorsPeter O. Krutzik, Garry Nolan
Life of this patent
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Abstract
We describe herein a cell-based multiplexing technique called detectable cell barcoding (DCB). In DCB, each individual sample is labeled with a different DCB signature that distinguishes each sample by one or both of detected intensity or type of detection characteristic. The samples are then combined and analyzed for a detectable characteristic of interest (e.g., presence of an analyte). By employing multiple distinct DCB labels at varying concentrations, one can perform multiplex analyses on up to hundreds or thousands (or more) of cell samples in a single reaction tube. DCB reduces reagent consumption by factors of 100-fold or more, significantly reduces data acquisition times and allows for stringent control sample analysis.
Claims28
A method of detecting a characteristic of cells in multiple cell samples, said method comprising:
- a) contacting multiple cell samples with a different amount of a first detectable cell barcode (DCB) label having a first detectable characteristic thereby generating multiple differentially labeled cell samples, wherein said first DCB label binds to the cells in said multiple cell samples;
- b) combining said multiple differentially-labeled cell samples to produce a combined cell sample;
- c) analyzing the cells in said combined cell sample to detect:
- i) the amount of said first detectable characteristic on said cells in said combined cell sample;
- and
- ii) at least one second detectable characteristic of said cells in said combined cell sample;
- to obtain a result, wherein detection of said first detectable characteristic does not interfere with detection of said at least one second detectable characteristic of said cells in said combined cell sample, and
- d) deconvoluting said result based on said detected amount of said first detectable characteristic on said cells to detect said at least one second detectable characteristic of said cells in each of said multiple cell samples.
Dependencies are inferred from the claim text.