Back to results
US 7,993,889 B1Granted
Fermentive production of four carbon alcohols
AssigneeButamax(Tm) Advanced Biofuels LLC
InventorsGail K. Donaldson, Andrew C. Eliot, Dennis Flint, Lori Ann Maggio-Hall, Vasantha Nagarajan
Life of this patent
Estimating the term…
Abstract
Methods for the fermentative production of four carbon alcohols is provided. Specifically, butanol, preferably isobutanol is produced by the fermentative growth of a recombinant bacterium expressing an isobutanol biosynthetic pathway.
Claims19
A method for producing isobutanol comprising;
- a. providing a fermentation media comprising carbon substrate;
- and
- b. contacting said media with a recombinant yeast microorganism expressing an engineered isobutanol biosynthetic pathway wherein said pathway comprises the following substrate to product conversions;
- i. pyruvate to acetolactate (pathway step a);
- ii. acetolactate to 2,3-dihydroxyisovalerate (pathway step b);
- iii. 2,3-dihydroxyisovalerate to α-ketoisovalerate (pathway step c);
- iv. α-ketoisovalerate to isobutyraldehyde (pathway step d);
- and
- v. isobutyraldehyde to isobutanol (pathway step e);
- and wherein
- a) the substrate to product conversion of step (i) is performed by an acetolactate synthase enzyme;
- b) the substrate to product conversion of step (ii) is performed by an acetohydroxy acid isomeroreductase enzyme;
- c) the substrate to product conversion of step (iii) is performed by an acetohydroxy acid dehydratase enzyme;
- d) the substrate to product conversion of step (iv) is performed by a decarboxylase enzyme;
- and
- e) the substrate to product conversion of step (v) is performed by an alcohol dehydrogenase enzyme;
- whereby isobutanol is produced.
14 more elements
Dependencies are inferred from the claim text.