Flow cytometer and flow cytometry
AssigneeBay Bioscience Kabushiki Kaisha
InventorMasahiko Kanda
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Abstract
In one embodiment of the present invention, a flow cytometer includes a plurality of optical sources, each of which illuminates an excitation beam at a predetermined frequency having a wavelength and a phase different from another; and a guide member for guiding the excitation beams into the same incident optical path to focus onto a dyed particle. According to one aspect of an embodiment of the present invention, it is possible, without requiring introduction of the delay time, to detect a plurality of fluorescence excited at the cell particles labeled with a plurality of fluorescent labeling reagents, by a plurality of laser optical sources.
Claims20
A flow cytometer, comprising:
- a plurality of optical sources, each of which illuminates an excitation beam at a predetermined frequency having a wavelength and a phase different from another;
- a guide member for guiding the excitation beams into the same incident optical path to focus onto a dyed particle running through a flow cell;
- a plurality of fluorescent detectors for detecting fluorescence from the particle excited by each of the excitation beams to output fluorescent signals;
- and
- a synchronizing/isolating circuitry for isolating the fluorescent signals in synchronization with each of the phases of the excitation beams into a plurality of optical signal components.
A flow cytometry, comprising:
- illuminating a plurality of excitation beams at a predetermined frequency having a wavelength and a phase different from another;
- guiding the excitation beams into the same incident optical path to focus onto a dyed particle running through a flow cell;
- detecting fluorescence from the particle excited by each of the excitation beams to output fluorescent signals;
- and
- isolating the fluorescent signals in synchronization with each of the phases of the excitation beams into a plurality of optical signal components.
Dependencies are inferred from the claim text.