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US 7,989,616 B2Granted
High-phosphate starch
AssigneeBayer CropScience AG
InventorsStephan Soyka, Claus Frohberg
Life of this patent
Estimating the term…
Abstract
This invention relates to modified starches having an elevated content of phosphate and an elevated content of amylose.
Claims19
A modified starch isolated from potato plants comprising
- an amylose content, as measured by the method of Hovenkamp-Hermelink et al. of between 40% and 50%,
- a phosphorus content of from 80 to 95 μmol of phosphate per gram of starch (dry weight), and
- an altered amylopectin side-chain distribution as compared with starch isolated from corresponding wild-type potato plants,
- wherein the proportion of side chains having a DP of less than 11 and/or a DP of from 11 to 18 is reduced, and the proportion of side chains having a DP of from 56 to 61 and a DP of from 62 to 123 is increased, as compared with starch isolated from corresponding wild-type potato plants, and
- wherein the proportion of the amylopectin side chains having a DP of from 62 to 123 is increased by at least 150%, based on the quantity of the amylopectin side chains having a DP of from 62 to 123 in starch isolated from corresponding wild-type plants.
2 more elements
Starch obtainable from a potato plant cell which exhibits an activity
- (a) of one or more SSIII proteins which occur endogenously in the plant cell;
- (b) of one or more BEI proteins which occur endogenously in the plant cell;
- (c) of one or more BEII proteins which occur endogenously in the plant cell;
- and
- (d) of one or more proteins which occur endogenously in the plant cell and which exhibit an at least 80% identity with the amino acid sequence of SEQ ID NO 12 or SEQ ID NO 14,
- which is reduced as compared with that of corresponding wild-type plant cells,
- wherein said starch comprises an altered amylopectin side-chain distribution as compared with starch isolated from corresponding wild-type potato plants, and
- further wherein the proportion of the amylopectin side chains having a DP of from 62 to 123 is increased by at least 150%, based on the quantity of the amylopectin side chains having a DP of from 62 to 123 in starch isolated from corresponding wild-type plants.
5 more elements
A plant cell or plant which exhibits an activity
- (a) of one or more SSIII proteins which occur endogenously in the plant;
- (b) of one or more BEI proteins which occur endogenously in the plant;
- (c) of one or more BEII proteins which occur endogenously in the plant;
- and
- (d) of one or more proteins which occur endogenously in the plant and which exhibit an at least 80% identity with the amino acid sequence of SEQ ID NO. 12 or SEQ ID NO. 14,
- which is reduced as compared with that of corresponding wild-type plants,
- wherein said plant cell or plant synthesizes a starch comprising an altered amylopectin side-chain distribution as compared with starch isolated from corresponding wild-type potato plants, and
- further wherein the proportion of the amylopectin side chains having a DP of from 62 to 123 is increased by at least 150%, based on the quantity of the amylopectin side chains having a DP of from 62 to 123 in starch isolated from corresponding wild-type plants.
5 more elements
A method of manufacturing starch comprising extracting starch from a potato plant cell, wherein said plant cell exhibits an activity
- (a) of one or more SSIII proteins which occur endogenously in the plant cell;
- (b) of one or more BEI proteins which occur endogenously in the plant cell;
- (c) of one or more BEII proteins which occur endogenously in the plant cell;
- and
- (d) of one or more proteins which occur endogenously in the plant cell and which exhibit an at least 80% identity with the amino acid sequence of SEQ ID NO 12 or SEQ ID NO 14,
- which is reduced as compared with that of corresponding wild-type plant cells,
- wherein said starch comprises an altered amylopectin side-chain distribution as compared with starch isolated from corresponding wild-type potato plants, and
- further wherein the proportion of the amylopectin side chains having a DP of from 62 to 123 is increased by at least 150%, based on the quantity of the amylopectin side chains having a DP of from 62 to 123 in starch isolated from corresponding wild-type plants.
5 more elements
Dependencies are inferred from the claim text.