Transgenic sweet sorghum with altered lignin composition and process of preparation thereof
AssigneeNagarjuna Energy Private Limited
InventorsAsitava Basu, Mrinal Kumar Maiti, Satarupa Kar, Soumitra Kumar Sen, Banibrata Pandey
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Abstract
The present invention provides a sweet sorghum plant characterized by altered lignin content and/or altered lignin composition compared to a wild plant and this is achieved by manipulating the expression of caffeoylCoA-O-methyltransferae (CCoAOMT) in particular and optionally caffeic acid-O-methyltranferase (COMT) in sweet sorghum by incorporation of a construct comprising an isolated DNA sequence represented by SEQ ID NO 2 and optionally SEQ ID NO 1.
Claims19
An isolated DNA sequence isolated from sweet sorghum (Sorghum bicolor) encoding Caffeoyl-CoA-O-methyltransferase (CCoAOMT), the sequence comprising SEQ ID NO 2.
A construct comprising an isolated nucleic acid sequence comprising SEQ ID NO 2, said nucleic acid being operably associated with a promoter, wherein the first nucleic acid is in the antisense orientation and is “antisense” to at least a portion of a CCoAOMT gene, and said construct optionally comprises a second nucleic acid represented by SEQ ID NO 1 that is an antisense to nucleotide sequence that encodes caffeic acid-O-methyltransferase (COMT).
A process for producing a transgenic sweet sorghum (Sorghum bicolor) plant having altered lignin content and composition, said process comprising:
- a. transfecting a sweet sorghum plant cell with a first isolated nucleic acid sequence comprising SEQ ID NO 2, and optionally a second isolated nucleic acid comprising SEQ ID NO 1, said nucleic acids associated with altered activity of a caffeoyl-CoA-O-methyltransferase (CCoAOMT) and caffeic acid-O-methyltranferase (COMT) in genetically engineered plants as compared to the activity of CCoAOMT and COMT in the wild plant, to yield a genetically engineered plant cell construct;
- and
- b. growing the plant cell into the transgenic sweet sorghum plant having altered lignin content as compared to the wild type plant.
Dependencies are inferred from the claim text.