Quantification of analytes using accelerator mass spectrometry
AssigneeXceleron Limited
InventorsRonald Colin Garner, Graham John Lappin
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Abstract
The invention provides a calibrating method for use in a method of determining the quantity of an analyte labeled with an accelerator mass spectrometry (AMS) isotope in a test sample. The samples can include, for example, urine, faeces, plasma or blood. The analyte can be, for example, a drug or metabolite of a drug.
Claims24
A calibrating method for use determining the quantity of an analyte labeled with an accelerator mass spectrometry isotope in a test sample, said calibrating method comprising:
- (i) contacting a plurality of samples contaminated with neither said analyte nor a non-labeled counterpart analyte with a known quantity of said counterpart analyte and a quantity C of said analyte to afford a plurality of calibrating samples, wherein each of said calibrating samples contains a known quantity of said counterpart analyte and a different quantity C of said analyte;
- (ii) measuring by accelerator mass spectrometry the quantity C of said analyte added to each of the plurality of samples;
- (iii) separating said analyte and said counterpart analyte from other species in the plurality of samples to afford a plurality of purified samples;
- (iv) measuring a quantity A of said analyte in said purified samples by accelerator mass spectrometry;
- and
- (v) measuring a quantity B of said counterpart analyte in said purified samples;
- and relating the measured quantities C to ratios of measured quantities A and B for each of the plurality of samples to allow for determination of the quantity of said analyte in said test sample.
A quantitative method for determining the quantity of an analyte labeled with an accelerator mass spectrometry isotope in a test sample, said method comprising:
- (i) contacting said test sample with a known quantity of a non-labeled counterpart analyte;
- (ii) separating said analyte, if present, and said counterpart analyte from other species in the test sample to afford a purified test sample;
- (iii) measuring a quantity A of said analyte in said purified test sample by accelerator mass spectrometry;
- (iv) measuring a quantity B of said counterpart analyte in said purified test sample;
- and
- (v) determining the quantity of analyte present is said test sample;
- wherein said quantitative method further comprises a calibrating method comprising:
- (a) contacting a plurality of samples contaminated with neither an analyte labeled with an accelerator mass spectrometry isotope nor a non-labeled counterpart analyte with a known quantity of said counterpart analyte and a quantity C of said analyte to afford a plurality of calibrating samples, wherein each of said calibrating samples contains a known quantity of said counterpart analyte and a different quantity C of said analyte;
- (b) measuring by accelerator mass spectrometry the quantity C of analyte added to each of the plurality of samples;
- (c) separating said analyte and said counterpart analyte from other species in the plurality of samples to afford a plurality of purified samples;
- (d) measuring a quantity A of said analyte in said purified samples by accelerator mass spectrometry;
- and
- (e) measuring a quantity B of said counterpart analyte in said purified samples;
- wherein the same analyte and counterpart analyte are employed in both the quantitative method and the calibrating method;
- and
- relating the measured quantities C to ratios of measured quantities A and B for each of the plurality of calibrating samples to allow for determination of the quantity of said analyte in said test sample.
Dependencies are inferred from the claim text.