Promoter sequence obtained from rice and methods of use
AssigneeArcadia Biosciences, Inc.
InventorsAllen G. Good, Mary DePauw, Ashok K. Shrawat
Life of this patent
Estimating the term…
Abstract
Methods are provided by which Oryza sativa plants and seeds thereof may be modified to express a coding region of interest using a promoter sequence operatively linked to the coding region. The promoter sequence is an isolated Oryza sativa antiquitin (OsAnt1) promoter sequence including SEQ ID NO: 1. The coding region of interest may encode a nitrogen utilization protein, suitably alanine aminotransferase. Methods to develop Oryza sativa plants that have increased biomass and seed yield are also presented. Furthermore, Oryza sativa plants may be produced that maintain a desired yield while reducing the need for high levels of nitrogen application.
Claims12
A method for directing expression of a coding region of interest encoding a target protein in an Oryza sativa plant comprising:
- contacting an Oryza sativa plant with a coding region of interest encoding a target protein in operative linkage with an isolated OsAnt1 promoter sequence consisting of SEQ ID NO:
- 1;
- and introducing into the plant the coding region of interest encoding said target protein in operative linkage with the OsAnt1 promoter sequence consisting of SEQ ID NO:
- 1, wherein the OsAnt1 promoter directs expression of the coding region of interest encoding said target protein in the plant.
A method of increasing biomass of an Oryza sativa plant, comprising:
- contacting an Oryza sativa plant with a coding region of interest encoding a target protein in operative linkage with an isolated OsAnt1 promoter sequence consisting of SEQ ID NO:
- 1;
- and introducing into the plant the coding region of interest encoding said target protein in operative linkage with the OsAnt1 promoter sequence consisting of SEQ ID NO:
- 1;
- wherein the OsAnt1 promoter directs expression of the coding region of interest encoding said target protein to obtain said Oryza sativa plant with increased biomass as compared to an Oryza sativa plant lacking said OsAnt1 promoter sequence.
A method for increasing seed yield of an Oryza sativa plant, comprising:
- contacting an Oryza sativa plant with a coding region of interest encoding a target protein in operative linkage with an isolated OsAnt1 promoter sequence consisting of SEQ ID NO:
- 1;
- and introducing into the plant the coding region of interest encoding said target protein in operative linkage with the OsAnt1 promoter sequence consisting of SEQ ID NO:
- 1, wherein the OsAnt1 promoter directs expression of the coding region of interest encoding said target protein to obtain said Oryza sativa plant with increased seed yield as compared to an Oryza sativa plant lacking said OsAnt1 promoter sequence.
Dependencies are inferred from the claim text.