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US 7,982,027 B2Granted
Lipid encapsulated interfering RNA
AssigneeProtiva Biotherapeutics, Inc.
InventorsIan MacLachlan, Ellen Grace Ambegia, James Heyes
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Abstract
The present invention provides compositions and methods for silencing gene expression by delivering nucleic acid-lipid particles comprising a siRNA molecule to a cell.
Claims63
A nucleic acid-lipid particle, said nucleic acid-lipid particle comprising:
- a chemically synthesized siRNA;
- a cationic lipid;
- a non-cationic lipid;
- and
- a conjugated lipid that inhibits aggregation of particles, wherein the conjugated lipid that inhibits aggregation of particles comprises a polyethyleneglycol-dialkyloxypropyl (PEG-DAA) conjugate having the following structure:
- wherein:
- R1 and R2 are independently selected and are alkyl groups having from about 10 to about 22 carbon atoms;
- PEG is a polyethyleneglycol, wherein the terminal hydroxyl group is substituted with a methyl group;
- and
- L is a carbamate linker moiety;
- and
- wherein said siRNA is fully encapsulated in said nucleic acid-lipid particle.
9 more elements
Dependencies are inferred from the claim text.