Ketoreductase polypeptides for the production of (R)-3-hydroxythiolane
AssigneeCodexis, Inc.
InventorsJack Liang, Stephane J. Jenne, Emily Mundorff, Rama Voladri, James Lalonde, Gjalt Huisman
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Abstract
The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize chiral compounds.
Claims39
An engineered ketoreductase polypeptide capable of stereoselectively reducing a 3-ketothiolane to (R)-3-hydroxythiolane with a percent stereomeric excess of at least 70%, which comprises an amino acid sequence that is at least 90% identical to a reference sequence based on SEQ ID NO:
- 4 and which has at the residue corresponding to X145 a serine.
Dependencies are inferred from the claim text.