Genetically stable enterovirus expression vector with replaced stem loop VI
AssigneeDuke University
InventorsMatthias Gromeier, Elena Y. Dobrikova
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Abstract
The present invention relates, in general, to an expression vector and in particular, to a genetically stable viral expression vector.
Claims11
An expression vector comprising an Enterovirus a portion of an internal ribosomal entry site (IRES) of which is replaced by a heterologous sequence encoding a protein,
- wherein a protein-encoding portion of said heterologous sequence replaces stem loop domain VI of said IRES and has the secondary structure, and thereby the function, of said replaced stem loop domain VI of said IRES, and
- wherein said heterologous sequence encoding said protein is up to 300 nucleotides in length.
A viral vector obtainable by replacing at least stem-loop domain VI of an IRES of an Enterovirus with a protein encoding portion of a heterologous sequence,
- wherein said protein-encoding portion of said heterologous sequence is a structural and functional mimic of said replaced stem-loop domain VI, and
- wherein said heterologous sequence is up to 300 nucleotides in length.
An expression vector comprising RPδ6 and a nucleic acid sequence encoding an antigen operably incorporated therein, wherein said nucleic acid sequence is up to 300 nucleotides in length.
Dependencies are inferred from the claim text.