Methods for production of xylitol in microorganisms
AssigneeBiotechnology Research and Development Corporation
InventorsPaul Taylor, Ian Fotheringham, Nathan Wymer, Badal Saha, David Demirjian, Yoshikiyo Sakaibara, Francis Michael Racine
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Abstract
The invention provides biosynthetic routes to xylitol production that do not require pure D-xylose for synthesis and that can utilize inexpensive substrates such as hemicellulose hydrolysates.
Claims35
A recombinant bacterium that expresses proteins comprising:
- (a) xylose specific reductase, wherein the xylose specific reductase is a Pichia stipitis reductase with Ser233Pro and Phe286Leu mutations;
- (b) L-arabitol dehydrogenase or ribitol dehydrogenase or both;
- and
- (c) L-xylulose reductase;
- that have a biochemical activity that produces xylitol from substrates comprising D-xylose;
- or L-arabinose;
- or L-arabinose and D-xylose, such that the recombinant bacterium produces and accumulates xylitol, and accumulates no substantial amount of L-arabitol;
- wherein the xylose reductase protein, or L-arabitol dehydrogenase protein, or ribitol dehydrogenase protein, or L-xylulose reductase protein, or a combination thereof are expressed from a recombinant polynucleotide.
An isolated recombinant microorganism that expresses a recombinant xylose specific reductase protein, wherein the xylose specific reductase protein is a Pichia stipitis reductase protein with Ser233Pro and Phe286Leu mutations.
A purified xylose specific reductase comprising SEQ ID NO:43.
A purified P. stipitis xylose reductase comprising a Ser233Pro mutation and a Phe286Leu mutation.
Dependencies are inferred from the claim text.