Method for obtaining new life forms
AssigneesInstitut Pasteur, Evologic GmbH, Rupert Mutzel, Centre National De La Recherche Scientifique
InventorsRupert Mutzel, Philippe Marliere, Didier Mazel
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Abstract
The present invention relates to a method for generating a novel form of life comprising the steps consisting of: a) irreversible alteration of the genome of a microbial clone; b) cultivation of a vast population of microbial cells originating from the altered clone obtained in step a) during numerous generations under conditions allowing selection for a higher and stable proliferation rate; c) isolation of descendant clones within the cultivated population of step b) still bearing the alteration of step a).
Claims15
A method for generating stable bacterial strains for producing non-formylated polypeptides comprising:
- a) inactivating a fmt gene coding for Met-tRNAi transformylase in the genome of at least one microbial clone to yield an altered clone,
- b) cultivating a population of microbial cells originating from the altered clone obtained in step a) under permanent proliferation in suspension allowing selection for a higher and stable proliferation rate;
- c) isolating descendant clones within the cultivated population of step b) still bearing the inactivation of step a).
Dependencies are inferred from the claim text.