# US 7,955,833 B2 · Animal protein free media for cultivation of cells

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- Number: US 7,955,833 B2
- Document ID: US7955833B2
- Status: Granted
- Kind: B2
- Application number: 10/614,037
- Filed: 2003-07-08
- Published: 2004-04-22
- Granted: 2011-06-07
- Earliest claimed: 2002-07-09
- Assignees: Baxter International Inc.; Baxter Healthcare S.A.
- Inventors: Manfred Reiter; Wolfgang Mundt; Leopold Grillberger; Barbara Kraus
- Web page: https://patentgrep.com/patent/US7955833B2

## CPC

- C12N 5/005: {Protein-free medium}
- A61K 39/12: Viral antigens
- A61K 39/145: Orthomyxoviridae, e.g. influenza virus
- A61K 39/275: Poxviridae, e.g. avipoxvirus
- A61K 39/285: Vaccinia virus or variola virus
- A61P 31/14: for RNA viruses
- A61P 31/16: for influenza or rhinoviruses
- A61P 37/02: Immunomodulators
- C12N 5/0043: {Medium free of human- or animal-derived components}
- C12N 7/00: Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof (preparing medicinal viral antigen or antibody compositions, e.g. virus vaccines, A61K39/00)
- C12N 2500/74: from fungi, e.g. yeasts
- C12N 2500/76: from plants
- C12N 2710/24134: Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
- C12N 2760/16134: Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
- C12N 2770/36134: Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
- Y02A 50/30: Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- Y02A: TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE

## Abstract

The present invention relates to animal protein free cell culture media comprising a combination of non-animal derived peptides derived from soy hydrolysate and yeast hydrolysate. The invention also provides an animal protein free culture process, wherein cells are cultivated, propagated and passaged without animal-derived components. This process is useful for cultivating cells, such as recombinant cells or cells infected with a virus, and for production biological products by cell culture processes under conditions devoid of animal protein components.

## Claims

### Claim 1

A method of producing an immunogenic composition comprising a virus or a virus antigen, wherein the method comprises:
a) providing a culture of VERO cells that have been grown in an animal protein free medium comprising soy hydrolysate at a concentration of about 0.05% (w/v) to about 1% (w/v) and yeast hydrolysate at a concentration of about 0.05% (w/v) to about 0.3% (w/v), wherein at least 90% of the hydrolysates have a molecular weight of less than or equal to 1000 Daltons;
b) infecting the cells with the virus;
c) incubating the infected cells to propagate the virus;
d) harvesting the virus or virus antigen produced; and
e) preparing an immunogenic composition from the harvested virus or virus antigen.

### Claim 2 (depends on 1)

The method according to claim 1, wherein said harvested virus or virus antigen is subjected to purification.

### Claim 3 (depends on 1)

The method according to claim 1, wherein the virus is selected from the group of orthomyxoviruses, paramyxoviruses, reoviruses, picornaviruses, flaviviruses, arenaviruses, herpesviruses, poxviruses, coronaviruses and adenoviruses.

### Claim 4 (depends on 1)

The method according to claim 1, wherein the harvested virus or virus antigen is purified by ion exchange or gel filtration.

### Claim 5 (depends on 1)

The method according to claim 1, wherein the virus is an influenza virus.