US 7,955,492 B2Granted

Method for measuring blood components and biosensor and measuring instrument for use therein

AssigneePanasonic Corporation

InventorsMasaki Fujiwara, Teppei Shinno, Shin Ikeda

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Abstract

The present invention provides a method of measuring a component in blood, by which the amounts of blood cells and an interfering substance can be measured with high accuracy and high reliability and the amount of the component can be corrected accurately based on the amounts of the blood cells and the interfering substance. In a sensor for measuring a blood component, a first working electrode 13 measures a current that flows during a redox reaction of a blood component, a second working electrode 17 measures the amount of blood cells, and a third working electrode 12 measures the amount of an interfering substance. Next, based on the measurement results, the amount of the blood component to be measured is corrected. Thus, more accurate and precise measurement of the amount of the blood component can be realized.

Claims25

Dependent claims

A method of measuring a component in blood, comprising the steps of:

measuring a component in blood by causing a redox reaction between the component and an oxidoreductase in the presence of a mediator, detecting a redox current caused by the redox reaction with a first electrode system comprising a working electrode and a counter electrode, and converting a value of the detected current into an amount of the component;
correcting the amount of the component using an amount of blood cells contained in the blood;
and
correcting the amount of the component using an amount of an interfering substance contained in the blood,
wherein the correction step using the amount of the blood cells comprises:
supplying the blood to a second electrode system comprising a working electrode and a counter electrode, a mediator being present on the counter electrode of the second electrode system but not on the working electrode of the second electrode system;
applying a voltage to the second electrode system in this state to cause a redox current to flow through the second electrode system;
detecting the redox current flowing through the second electrode system;
converting a value of the detected redox current flowing through the second electrode system into the amount of the blood cells;
and
correcting the amount of the component based on the amount of the blood cells, and the correction step using the amount of the interfering substance comprises:
supplying the blood to a third electrode system comprising a working electrode and a counter electrode;
applying a voltage to the third electrode system in this state to cause a redox current to flow through the third electrode system;
detecting the redox current flowing through the third electrode system;
converting a value of the detected redox current flowing through the third electrode system into the amount of the interfering substance;
and
correcting the amount of the component based on the amount of the interfering substance.

14 more elements

A biosensor for measuring a component in blood by causing a redox reaction of the component and detecting a redox current caused by the redox reaction with an electrode,

wherein the biosensor comprises:
a first analysis portion comprising a first electrode system on which at least an oxidoreductase that acts upon the component and a mediator are provided;
a second analysis portion comprising a second electrode system that comprises a working electrode and a counter electrode, a mediator being provided on the counter electrode but not on the working electrode;
and
a third analysis portion comprising a third electrode system that comprises a working electrode and a counter electrode,
in the first analysis portion, the component in the blood is measured by causing a redox reaction between the component and the oxidoreductase in the presence of the mediator and detecting with the first electrode system a redox current caused to flow when a voltage is applied,
in the second analysis portion, an amount of blood cells contained in the blood is measured by supplying the blood to the second electrode system, applying a voltage to the second electrode system in this state to cause a redox current to flow through the second electrode system, and detecting the redox current, and
in the third analysis portion, an amount of an interfering substance contained in the blood is measured by supplying the blood to the third electrode system, applying a voltage to the third electrode system in this state to cause a redox current to flow through the third electrode system, and detecting the redox current.

5 more elements

Dependencies are inferred from the claim text.

Facts

Application: 10/598,001Filed 18 Apr 2005
Priority: JP 2004-12322019 Apr 2004
Published: US 2007/0138026 A121 Jun 2007
Granted: 7 Jun 2011
Term ends: Not estimatedThe source records don’t support an estimate.

Classification12 codes

G01N 27/3272
Amperometric enzyme electrodes for analytes in body fluids, e.g. glucose in blood: test elements therefor, i.e. disposable laminated substrates…
A61B 5/14546
Measuring characteristics of blood in vivo, e.g. gas concentration or pH-value ; Measuring characteristics of body fluids or tissues, e.g.…: for…
A61B 5/1486
Using chemical or electrochemical methods, e.g. by polarographic means: using enzyme electrodes, e.g. with immobilised oxidase
C12Q 1/004
Enzyme electrodes: mediator-assisted