Delta 4,5 glycuronidase compositions and methods related thereto

A method for the recombinant production of substantially pure Δ4, 5 glycuronidase, comprising:

• a) introducing into a host cell an isolated nucleic acid molecule encoding a polypeptide having Δ4, 5 glycuronidase activity, wherein the isolated nucleic acid molecule is selected from the group consisting of:
• (i) nucleic acid molecules which hybridize under stringent conditions to a nucleic acid molecule having a nucleotide sequence set forth as SEQ ID NO:2, wherein the hybridization conditions are hybridization at 65° C. in hybridization buffer comprising 3.5× (0.15M sodium chloride/0.015M sodium citrate, pH7), 0.02% Ficoll, 0.02% polyvinyl pyrrolidone, 0.02% bovine serum albumin, 25 mM NaH2PO4, pH 7, 0.5% sodium dodecylsulphate and 2 mM ethylene diamine tetra acetic acid,
• (ii) nucleic acid molecules that differ from the nucleic acid molecules of (i) in codon sequence only due to degeneracy of the genetic code, and
• (iii) full complements of (i) or (ii);
• b) expressing recombinant Δ4, 5 glycuronidase in the host cell;
• and
• c) isolating the recombinant Δ4, 5 glycuronidase from the host cell to produce substantially pure Δ4, 5 glycuronidase.