Methods and organisms for the growth-coupled production of 1,4-butanediol
AssigneeGenomatica, Inc.
InventorsAnthony P. Burgard, Stephen J. Van Dien, Mark Burk
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Abstract
The invention provides a non-naturally occurring microorganism comprising one or more gene disruptions, the one or more gene disruptions occurring in genes encoding an enzyme obligatory to coupling 1,4-butanediol production to growth of the microorganism when the gene disruption reduces an activity of the enzyme, whereby the one or more gene disruptions confers stable growth-coupled production of 1,4-butanediol onto the non-naturally occurring microorganism. The microorganism can further comprise a gene encoding an enzyme in a 1,4-butanediol (BDO) biosynthetic pathway. The invention additionally relates to methods of using microorganisms to produce BDO.
Claims12
A non-naturally occurring Escherichia coli microorganism comprising a set of metabolic modifications, said set of metabolic modifications comprising disruption of genes encoding alcohol dehydrogenase, pyruvate formate lyase, lactate dehydrogenase and malate dehydrogenase, wherein said E. coli microorganism expresses heterologous enzymes that provide production of 1,4-butanediol.
Dependencies are inferred from the claim text.