Biosynthesis of phloroglucinol and preparation of 1,3-dihydroxybenzene therefrom
AssigneeBoard of Trustees of Michigan State University
InventorJohn W. Frost
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Abstract
The present invention provides methods, enzymes, and cells for the biosynthetic production of phloroglucinol from malonyl-CoA, which is ultimately obtained from simple starting materials such as glucose; also provided are methods for preparing derivatives of biosynthetic phloroglucinol, including, e.g., resorcinol.
Claims12
A recombinant cell comprising a phloroglucinol synthase having the amino acid sequence of SEQ ID NO:
- 2, wherein the recombinant cell does not express other active enzymes involved in the acetylphloroglucinol synthesis pathway, the recombinant cell being capable of converting malonyl-CoA to phloroglucinol.
An isolated or recombinant nucleic acid vector comprising at least one open reading frame encoding a phloroglucinol synthase having the amino acid sequence of SEQ ID NO:
- 2, wherein said nucleic acid vector does not express at least one of other active enzymes involved in the acetylphloroglucinol synthesis pathway, and further comprises at least one open reading frame encoding an enzyme of the malonyl-CoA synthesis pathway.
A process for the preparation of a recombinant cell that is capable of biosynthesizing phloroglucinol from malonyl-CoA, comprising transforming a cell with a nucleic acid encoding a phloroglucinol synthase having the amino acid sequence of SEQ ID NO:
- 2, wherein the recombinant cell does not express other active enzymes involved in the acetylphloroglucinol synthesis pathway.
Dependencies are inferred from the claim text.