# US 7,939,344 B2 · Use of particulate labels in bioanalyte detection methods

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- Number: US 7,939,344 B2
- Document ID: US7939344B2
- Status: Granted
- Kind: B2
- Application number: 12/188,976
- Filed: 2008-08-08
- Published: 2009-09-03
- Granted: 2011-05-10
- Earliest claimed: 2003-11-05
- Assignees: Trellis Bioscience, LLC
- Inventors: Lawrence M. Kauvar; Remy Cromer; William D. Harriman; Ellen J. Collarini
- Web page: https://patentgrep.com/patent/US7939344B2

## CPC

- G01N 15/0625: {Optical scan of the deposits}
- G01N 21/6428: {Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" (in vivo A61B5/00; immunoassay G01N33/53)}
- G01N 21/6458: {Fluorescence microscopy (fluorescence microscopes per se G02B21/0076 and G02B21/16)}
- G01N 27/44726: {using specific dyes, markers or binding molecules}
- G01N 30/88: Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
- G01N 33/505: {involving T-cells}
- G01N 33/5052: {involving B-cells}
- G01N 33/54313: {the carrier being characterised by its particulate form}
- G01N 33/585: {with a particulate label, e.g. coloured latex}
- G01N 30/74: Optical detectors {(measurement of intensity, velocity, spectral content, polarisation, or phase of infrared, visible or ultraviolet light G01J)}
- G01N 2021/6441: {with two or more labels}
- G01N 2030/8813: {biological materials}
- G01N 2030/8827: {involving nucleic acids}
- Y10T 436/112499: with sample on test slide
- Y10T 436/13: Tracers or tags
- Y10T 436/25125: Digestion or removing interfering materials
- Y10T 436/25375: Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)
- Y10T: TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION

## Abstract

New applications for the use of distinguishable particulate labels available in a variety of hues and sized in the submicron range are described. These applications include profiling of cellular components, obtaining secretion patterns, identifying a multiplicity of components in chromatographic or electrophoretic techniques and identification of desired immunoglobulin secreting cells.

## Claims

### Claim 1

A method to identify and recover cells contained within a cell sample that secrete an immunoglobulin of desired specificity and affinity which method comprises:
(a) providing onto a sample surface a multiplicity of cell samples, each of which cell samples contains cells that secrete immunoglobulins onto the sample surface, said surface optionally comprising a capture reagent for immunoglobulins, wherein each cell sample is dispensed onto a specified location on the sample surface to which the cells secrete the immunoglobulins;
(b) probing said locations on the sample surface with a multiplicity of epitopes and/or antigens each labeled with a distinguishable particulate label wherein said multiplicity includes desired epitopes and/or antigens and undesired epitopes and/or antigens to which the immunoglobulins bind;
(c) selecting any location of the surface which comprises immunoglobulins that bind to desired epitopes and/or antigens, but not to undesired epitopes and/or antigens;
(d) identifying the cell sample having the cells which secrete immunoglobulins at the selected location; and
(e) recovering the identified cell sample.

### Claim 2 (depends on 1)

The method of claim 1, wherein said cell samples are samples of single cells or progeny thereof.

### Claim 3 (depends on 1)

The method of claim 1, wherein said cells are B-cells derived from spleen, lymph nodes, mucosal lymph, or peripheral blood.

### Claim 4 (depends on 1)

The method of claim 1 wherein each cell sample is supported on a membrane that is permeable to immunoglobulins but not to cells.