Method and apparatus for substantially isolating plant tissues
AssigneeMonsanto Technology LLC
InventorsWhitney R. Adams, Jr., Brandon Davis, Lubomyr Kucher, Brian Martinell, Jyoti Rout, Brenda Lowe
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Abstract
The present invention provides methods and devices for the rapid isolation of monocot plant embryos suitable for transformation or tissue culture. The invention includes mechanical devices for substantially isolating plant embryos for use as transformable explants. Media suitable for isolating plant embryos and methods for their preparation are also provided.
Claims8
A method for obtaining embryos suitable for tissue culture and/or genetic transformation, comprising at least partially excising an embryo from a plant seed in a liquid medium consisting essentially of water and/or an osmotic agent with an osmolality of about 0 mOsm/kg to about 600 mOsm/kg, wherein the embryo remains viable for tissue culture and/or genetic transformation following excising the embryo from the plant seed;
- further comprising the step of genetically transforming the embryo, wherein the step of transforming comprises use of a co-culture medium comprising a bactericide, and wherein the co-culture medium comprises about 0.5-1.5 mg/L of 2,4-D.
Dependencies are inferred from the claim text.