US 7,939,250 B2 · Vitamin K epoxide recycling polypeptide VKORC1, a therapeutic target of coumarin and their derivatives

Abstract

The invention relates to a novel polypeptide vitamin K epoxide recycling polypeptide (VKORC1) as a target for coumarin and its derivatives. The invention further provides methods for identifying coumarin derivatives, and also claims VKORC1 polypeptides and VKORC1 nucleic acids containing a sequence abnormality associated with a VKORC1 associated deficiency such as warfarin resistance, wherein the VKORC1 polypeptides and VKORC1 nucleic acids can be used for diagnosing these deficiencies. Moreover, the invention relates to methods for identifying coumarin derivatives usable in pest control of rodents.

Claims4

Dependent claims

A method of diagnosing warfarin resistance in a patient comprising the steps of:

(I) amplifying a DNA sample obtained from the patient or reverse transcribing an RNA sample obtained from the patient into a DNA and amplifying the DNA;
and
(II) analyzing the amplified DNA of step (I) to determine at least one sequence mutation in an amino acid sequence of the vitamin K epoxide recycling (VKORC1) polypeptide as compared to the VKORC1 sequence set forth in SEQ ID NO:
1 wherein the presence of a mutation in the sequence of SEQ ID NO:
1 in the VKORC1 protein of said patient is indicative of the patient being warfarin resistant;
wherein said mutation in the amino acid sequence set forth in SEQ ID NO:
1 is selected from the group consisting of V29L, R98W, and Y139C.

4 more elements

Dependencies are inferred from the claim text.

Facts

Application

10/965,694Filed 14 Oct 2004 · us provisional application

Priority

14 Oct 2003

Published

US 2005/0271644 A18 Dec 2005

Granted

10 May 2011

Term ends

Not estimatedThe source records don’t support an estimate.

Classification23 codes

C12N 9/0006
Oxidoreductases: acting on CH-OH groups as donors
A01K 67/0276
Genetically modified vertebrates, e.g. transgenic: knock-out vertebrates
C07K 16/40
Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies: against enzymes
C12N 15/1137
Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming…: against enzymes

Vitamin K epoxide recycling polypeptide VKORC1, a therapeutic target of coumarin and their derivatives

Life of this patent

Vitamin K epoxide recycling polypeptide VKORC1, a therapeutic target of coumarin and their derivatives

Life of this patent

Abstract

Claims4