Antibody that binds human eosinophil-derived basic protein

A method of making a polyclonal antibody, the method comprising:

• a) immunizing an animal with a polypeptide comprising the an amino acid sequence of SEQ ID NO:
• 1, under conditions to elicit an antibody response;
• and
• b) purifying the antibodies with a polypeptide consisting of SEQ ID NO:
• 1, thereby identifying a polyclonal antibody that binds specifically to the amino acid sequence of SEQ ID NO:
• 1.

A method of making a monoclonal antibody, comprising:

• a) immunizing an animal with a polypeptide comprising the amino acid sequence of SEQ ID NO:
• 1, under conditions to elicit an antibody response;
• b) isolating antibody producing cells from the animal;
• c) fusing the antibody producing cells with immortalized cells in culture to form monoclonal antibody-producing hybridoma cells;
• d) culturing the hybridoma cells;
• and
• e) isolating from the culture monoclonal antibodies which bind specifically to a polypeptide consisting of the amino acid sequence of SEQ ID NO:
• 1.