Recombinant production of mixtures of antibodies
AssigneeMerus N.V.
InventorsPatrick H. C. Van Berkel, Ronald Hendrik Peter Brus, Ton Logtenberg, Abraham Bout
Life of this patent
Estimating the term…
Abstract
The invention provides methods for producing mixtures of antibodies from a single host cell clone, wherein, a nucleic acid sequence encoding a light chain and nucleic acid sequences encoding different heavy chains are expressed in a recombinant host cell. The recombinantly produced antibodies in the mixtures according to the invention suitably comprise identical light chains paired to different heavy chains capable of pairing to the light chain, thereby forming functional antigen-binding domains. Mixtures of the recombinantly produced antibodies are also provided by the invention. Such mixtures can be used in a variety of fields.
Claims17
A composition comprising three or more non-identical antibodies and a pharmaceutically acceptable carrier,
- wherein said three or more non-identical antibodies comprise at least three antibodies comprising heavy chains having different immunoglobulin heavy chain sequences and one common light chain,
- wherein at least three different heavy chain sequences are represented in the composition, and wherein the common light chain is paired with the at least three immunoglobulin heavy chains having different immunoglobulin heavy chain sequences to form functional antigen binding domains, and
- wherein the three or more non-identical antibodies comprise constant regions of isotypes selected from the group consisting of IgG, IgA, IgD, IgE, IgM, and mixtures of any thereof.
A composition comprising three or more non-identical antibodies and a pharmaceutically acceptable carrier, wherein the three or more non-identical antibodies comprise at least three antibodies comprising heavy chains having different immunoglobulin heavy chain sequences and one common light chain, wherein at least three different heavy chain sequences are represented in the composition, and wherein the common light chain is paired with the at least three immunoglobulin heavy chains having different immunoglobulin heavy chain sequences to form functional antigen binding domains, and
- wherein said three or more non-identical antibodies comprise at least one bispecific antibody.
Dependencies are inferred from the claim text.